Genome sequence, transcriptome, and annotation of rodent malaria parasite Plasmodium yoelii nigeriensis N67

Abstract Background Rodent malaria parasites are important models for studying host-malaria parasite interactions such as host immune response, mechanisms of evasion killing, and vaccine development. One the rodent is Plasmodium yoelii , multiple P. strains or subspecies that cause different disease phenotypes have been widely employed in various studies. The genomes transcriptomes several analyzed annotated, including lethal y. YM (or 17XL) non-lethal 17XNL/17X. Genomic DNA sequences cDNA reads from another nigeriensis N67 reported studies genetic polymorphisms response to drugs, but its genome has not assembled annotated. Results We performed sequencing using PacBio long-read technology, de novo transcriptome, predicted 5383 genes with high overall annotation quality. Comparison annotated those 17X revealed a set N67-specific orthology, expansion gene families, particularly homologs chabaudi erythrocyte membrane antigen, large numbers SNPs indels, proteins interact responses based on their functional domains. Conclusions highly diverse, having approximately one polymorphic site per 50 base pairs DNA. transcriptome provide searchable databases fast retrieval proteins, which will greatly facilitate our efforts biology function developing effective control measures against malaria.